Paloma Dutta Molecular biologist
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HI, I am a molecular biologist . I have experience in working in cutting edge research lab. I have expertise in different molecular biology techniques such as RTPCR, SANGER SEQUENCING, NGS, PROTEIN BIOLOGY. I have 10 years teaching experience. I am available for online teaching.
HI, I am a molecular biologist . I have experience in working in cutting edge research lab. I have expertise in different molecular biology techniques such as RTPCR, SANGER SEQUENCING, NGS, PROTEIN BIOLOGY. I have 10 years teaching experience. I am available for online teaching.
Thank you

Subjects

  • Molecular Biology Expert

Experience

  • SENIOR MOLECULAR TECHNOLOGIST (Mar, 2023–Mar, 2024) at KARKINOS HEALTHCARE
    Development and implementation of SOPS for
    molecular oncology lab
    • Initiated and standardized of RT PCR and
    sequence-based assay for cancer diagnosis
    • Performing Whole Genome Sequencing,
    targeted gene panel sequencing in Illumina
    Novaseq and Nextseq Sequencer
    • Laboratory inventory management as per
    institute inventory management policy.
  • SCIENTIFFIC OFFICER (Jan, 2021–Feb, 2023) at TATA MEDICAL CENTER, KOLKATA
    Standardization of different Real Time PCR, DD
    PCR assay for molecular oncology test
    development
    • Performing routine DNA and RNA extraction
    from blood and FFPE tissue for various
    molecular assay. RT-PCR, Fragment Analysis,
    Sanger sequencing, NGS of clinical samples
    • Analyzing RT PCR, DD PCR, sequencing results
    and generation of test reports for single gene
    and investigational test.
  • JRF (Sep, 2018–Dec, 2019) at Amity university
    • Assessment of different EMT markers expression
    at both RNA and protein level is done using
    NMUMG cells as a model system
    • Analysis of RNAseq data which intoned
    changes at the transcriptional level during
    induction of EMT by TGF-beta exposure
    • The RNAseq data was then further validated
    by real time PCR analysis and expression of
    those genes at protein level also checked by
    Western Blotting.
  • RESEARCH ASSISTANT (Feb, 2018–Aug, 2018) at IISER kolkata
    In this project, I have performed protein (BTK)
    purification using NI-NTA column by HPLC
    method
    • After purifying protein, I have done Gel filtration
    assay for deducing the interaction between BTK
    and IP6.

Education

  • MSC IN MOLECULAR BIOLOGY AND BIOTECHNOLOGY (Sep, 2015–Aug, 2017) from University of Kalyani, Kalyani in West Bengal–scored 62.81 %

Fee details

    ₹500–1,500/hour (US$5.85–17.55/hour)

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