JRF (Jan, 2018
–Jan, 2019) at IISER Bhopal
Working at the Molecular Biophysics Laboratory in IISER, I primarily acquired knowledge in molecular biology, Genetic engineering, and biophysical techniques. I worked with mitochondrial outer membrane proteins (mOMPs) such as VDAC and BAM, which play additional roles in ROS suppression, aging, cancer, and neurodegenerative diseases. I studied the biogenesis, function, and regulation of these proteins. Our ultimate goal is to identify a functional link between mitostasis, mOMP remodeling, and quality control and the onset of cancer, neurodegeneration, and aging-associated diseases. During that time, I was gained experience in cloning, ASM mutant library creation, Sample preparations for sequencing, Primer designing,Bacterial and Yeast transformations, Proteins expression, extraction and purification, Proteins folding and unfolding kinetics, equilibrium studies etc.